| 摘要 |
<p>The invention relates to a process for the chromatographic determination of ions, particularly anions on a reversed-phase column, in which sample ions are introduced into a mobile phase containing a modifier and a counterion, the mobile phase with the modifier, counterion and sample ions is passed through the column and, after separation with the aid of a suitable detection method, preferably an indirect UV-detection method, the individual sample ions are determined quantitatively via the decrease the counterion concentration in the eluate. In this process the mobile phase is a mixture of water and an organic solvent and has a pH-value of more than 5. The modifier used is a quaternary ammonium hydroxide of formula <CHEM> in which at least one radical is a straight-chained or branched alkyl radical with at least 8 and up to 20, more particularly 12 to 18 C-atoms. Prior to the passage of the mobile phase containing the sample ions, the reversed-phase column is preferably brought into equilibrium with the modifier, particularly with the modifier and the counterion. Trimethyl hexadecyl ammonium hydroxide is preferable used as the quaternary ammonium hydroxide. The invention also relates to the use of said quaternary ammonium hydroxides for the quantitative chromatographic determination of ions and in particular for the determination of inorganic anions, as well as to a reversed-phase material chromatographic column obtained through the conditioning with the modifier.</p> |
