MONOCLONAL ANTIBODY AGAINST BLOOD COAGULATION FACTOR XIII

摘要

PURPOSE:To enable quick, easy and accurate determination of the subject factor by culturing a specific hybridoma. CONSTITUTION:Subunit (a) of blood coagulation factor XIII (A) is prepared by fractionating and purifying human plasma, etc. An antibody-producing cell (B) is produced by immunizing mouse BALB/c with the component A and separating the spleen cell from the mouse. The cell B is mixed with mouse myeloma cell (C) such as SP2/OAg14 at a ratio of 1:(10-1X10<7-8>), subjected to cell fusion in the presence of polyethylene glycol and screened and cloned to obtain a hybridoma (D) capable of producing monoclonal antibody. The objective monoclonal antibody (E) against the component A is produced by culturing the strain D and purifying the cultured product by ammonium sulfate fractionation, ion exchange chromatography, etc. The antibody E optionally labeled with an enzyme such as peroxidase and the antibody E adsorbed to a microplate are made to react with desired antigen and the blood coagulation factor XIII is determined by enzyme immunoassay.