Process for producing biologically active plasminogen activator in recombinant CHO cells using suspension culture and removing detrimental components from medium

摘要

A method of producing biologically active human tissue plasminogen activator in suspension culture is provided wherein recombinant Chinese hamster ovary cells are cultured and certain components detrimental to recovery and biological activity are removed as a cell-free filtrate by cross-flow filtration. The human tissue plasminogen activator is recovered from the culture medium.