发明名称 |
HYBRIDIZATION METHOD AND PROBE |
摘要 |
In order to carry out hybridization reactions by forming a complex between a polynucleotide and a detectable substance and using the complex thus formed for hybridization reactions, a single strand polynucleotide is reacted with a positively charged nucleic acid binding detectable protein or polyamine in solution and the resultant complex is covalently bound with a cross-linking agent and the polynucleotide compound obtained is used as a marker in hybridization experiments in the elucidation of complementary sequences in foreign polynucleotides. The protein may be an enzyme such as horseradish peroxidase modified by a polyamine e.g. a polyethylene imine to provide a positively charged region. Assay sensitivity may be enhanced by performing the hybridization in the presence of about 6% (w/v) of a polyethylene glycol.
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申请公布号 |
US5053326(A) |
申请公布日期 |
1991.10.01 |
申请号 |
US19890724307 |
申请日期 |
1989.04.17 |
申请人 |
EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL) |
发明人 |
RENZ, MANFRED |
分类号 |
C12N15/09;C07H21/00;C07K1/107;C07K14/415;C12N9/00;C12N15/00;C12Q1/28;C12Q1/42;C12Q1/68;G01N33/50;G01N33/58 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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