发明名称 |
DNA SEQUENCING |
摘要 |
<p>A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.</p> |
申请公布号 |
GR900300159(T1) |
申请公布日期 |
1991.09.27 |
申请号 |
GR19900300159T |
申请日期 |
1991.09.27 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
TABOR, STANLEY;RICHARDSON, CHARLES C. |
分类号 |
C12Q1/42;C12Q1/48;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/42 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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