CLONING AND EXPRESSION OF RESTRICTION ENDONUCLEASES FROM NEISSERIA

摘要

The present invention is directed to a restriction endonuclease which recognizes and cleaves the palindromic sequence (I) between the first G and C residues from the 5' end, producing a four-base 5' extension. Also provided in this invention is a modification methylase corresponding to said restriction endonuclease. One source of these enzymes is Neisseria gonorrhoeae. This invention is also concerned with cloning and expressing the genes coding for said restriction endonuclease and said modification methylase.