| 摘要 |
PURPOSE:To obtain human PDI in high efficiency by incubating in a medium Bacillus brevis containing a specific DNA. CONSTITUTION:Firstly, Bacillus brevis H 102 strain is extracted and separated to produce (A) a DNA (e.g. plasmid pNU 200) containing, at its 3' end, a region coding signal peptide, and also containing a promoter region. Second, from human placental cDNA library, a cloning is made to produce (B) human protein disulfide isomerase(PDI). Third, a DNA coding the human PDI is bound to the 3' end of the component A to produce (C) plasmid pNU 200-PDI. Fourth, a (D) transformant produced by introducing the component C into Bacillus brevis H 102 strain is incubated in a medium containing glucose, etc., at pH5-9 at 10-42 deg.C for 10-166hr into (E) a cultured product. finally, bacteria produced by centrifugation of the component E is extracted and separated to collect the objective human PDI. |
