DETECTION OF POINT MUTATIONS IN GENES ENCODING GTP BINDING PROTEINS

摘要

The present invention provides a method for detecting whether a point mutation is present in a nucleic acid encoding a GTP bindingprotein or protein subunit. Methods are also provided for characterizing point mutations, if present. In a preferred embodiment, the method involves amplification of a nucleic acid segment followed by sequence-specific probe hybridization. The method is preferred for nucleic acids which encode a G-protein alpha subunit or p21 ras protein.