发明名称 |
METHOD OF PURIFYING RECOMBINANT POLYPEPTIDES |
摘要 |
A method of purifying a desired polypeptide and a kit for practicing the same are disclosed. The method comprises producing a fusion protein comprising a desired polypeptide linked to an affinity peptide that contains an endopeptidase cleavage site. An immobilized endopeptidase is used to separate fusion proteins from impurities and cleave the fusion protein into its substituent parts. |
申请公布号 |
AU7166891(A) |
申请公布日期 |
1991.08.21 |
申请号 |
AU19910071668 |
申请日期 |
1991.01.11 |
申请人 |
THE UPJOHN COMPANY |
发明人 |
SATISH K SHARMA;DAVID B EVANS |
分类号 |
C07K14/52;C07K1/12;C07K1/14;C07K14/48;C07K14/54;C07K14/545;C07K19/00;C12N9/00;C12N9/12;C12N9/72;C12N15/09;C12N15/62;C12P21/00;C12P21/02;C12P21/06;C12R1/19 |
主分类号 |
C07K14/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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