SEPARATION OF SALIVARY GLAND CELL OF MITE AND PROCESS FOR COLLECTING SPOROZOITE FROM THE CELL

摘要

PURPOSE:To recover sporozoite from a salivary gland cell of micro-piroplasma protozoa by extracting the salivary gland of a mite infected with a micro- piroplasma protozoa and treating the gland with a digestive enzyme at a low concentration to separate the salivary gland cells. CONSTITUTION:The salivary gland of a mite infected with a micro-piroplasma protozoa is extracted and treated with a digestive enzyme (preferably trypsin) having low concentration (preferably 0.01-0.1%) at 2-6 deg.C to separate the salivary gland cells. The cells are disintegrated to recover the sporozoite of the micro- piroplasma protozoa in living state.