| 摘要 |
<p>NEW MATERIAL:A hybridoma obtained by fusing a human myeloma cell strain (RPM 18226) with a mouse myeloma cell strain (FO), subsequently making the fused cell resistant to 8-azaguanine, and further repeating cloning and having following properties; Efficiency of fusion with human lymphatic corpuscle is high. The hybridoma itself dose not produce an antibody. An hybridoma between human lymphatic corpuscle has a high efficiency of cloning, can produce a large amount of IgG antibody and can proliferate thoroughly even in a serum- free medium. USE:Production of human monoclonal antibody. PREPARATION:For example, a human myeloma cell strain (RPM 18226) is fused with an antibody-nonproductive mouse myeloma cell (FO) having a high fusion efficiency using polyethylene glycol, etc., and the resultant fused cell is subsequently cultured in an ouabain-containing HAR culture medium to obtain a hybridoma. The resultant hybridoma is then subjected to cloning using the limiting dilution method, etc., thus obtaining the objective hybridoma capable of production of human monoclonal antibody.</p> |
