SYNTHETIC PEPTIDES AS MODULATORS OF FUNCTIONAL RESPONSES OF INTACT CELLS

摘要

The purpose of this invention is to provide methods for selectively inhibiting the INTRACELLULAR biochemical processes that lead to cellular responses through use of specifically designed synthetic peptide substrates (pseudosubstrates) or inhibitors of protein kinases or peptides that interfere with the action of DNA-binding proteins. These synthetic peptides will block activities of enzymes by competing with natural substrates or by preventing functioning of DNA binding proteins. This invention provides a novel means of modulation of intact cells by using specifically designed synthetic peptides which serve as substrates (pseudosubstrates) or inhibitors of protein kinases which are able to strongly inhibit activation of unpermeabilized cells. Functional responses of lymphocytes DOLLAR (i) in vitro as appropriate model systems are particularly illustrated.