发明名称 |
Process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids |
摘要 |
The invention relates to a process for the conversion of corrinoids produced by microorganisms into cyanocorrinoids by reaction with cyanides. According to the invention a fermentation broth obtained by disruption of microorganism cells in a known manner, preferably by heat treatment in the presence or absence of sulfite ions, optionally after purification steps known per se, is contacted with a suitable adsorbent or ion exchange resin, the corrinoids adsorbed on the surface of the adsorbent or ion exchange resin are treated with an aqueous solution containing cyanide ions or a compound capable of supplying cyanide ions, in an amount providing cyanide ions in a 1.1 to 2.0-fold molar excess related to the corrinoids, the adsorbent or ion exchange resin is washed with water, and the cyanocorrinoids obtained is eluted in a known manner, preferably with aqueous ethanol.
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申请公布号 |
US5026640(A) |
申请公布日期 |
1991.06.25 |
申请号 |
US19840657748 |
申请日期 |
1984.10.04 |
申请人 |
RICHTER GEDEON VEGYESZETI GYAR RT |
发明人 |
KELEMEN, AGNES;CSEREY PECHANY, EVA;JAKSA, ISTVAN |
分类号 |
C07D487/22;C07H23/00;C12P17/18;C12P19/42;C12R1/01;C12R1/15;C12R1/225 |
主分类号 |
C07D487/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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