| 摘要 |
<p>This application describes a process for producing des(64,65)-human proinsulin from human proinsulin by treating the latter with trypsin and carboxypeptidase B. The process is conducted by applying any or all of the following parameters: (1) use of an alkylated or acylated trypsin; (2) a temperature of from about 0 DEG C. to about 10 DEG C; (3) a pH of from about 8 to about 10; and (4) use of a buffer selected form the group consisting of ammonium bicarbonate and glycine.</p> |
