| 摘要 |
Specific binding assays for measuring the concentration of an analyte in a sample involve forming a complex comprising a solid support, the analyte or an analogue of the analyte, and an enzyme. The enzyme is used to cleave non-isotopic labels that are directly detectable from a substrate/label conjugate to provide a plurality of released labels in the free state, which are then separated from conjugate containing unreleased label. The released labels are detected for measuring the concentration of the analyte in the sample. Preferably the analyte or analogue is connected to the solid support by a cleavable bond, so that the enzyme can be separated from the solid support for use in the enzymatic cleaving of labels from the conjugate. This preferred version reduces the adverse effect of non-specific binding on the sensitivity and accuracy of the assay. |
