The use of moranoline and its derivatives as stabilizing agent for enzymes.

摘要

In accordance with a first aspect, the invention provides a process for producing an N-substituted moranoline derivative of the formula: (wherein R is a hydrogen atom or a C1-C5 alkyl group) comprising the steps of:- (a) adding a glucose donor to a culture broth obtained by culturing moranoline-producing bacteria as it is or having been subjected to alkylation, and then reacting cyclodextrin glycosyl transferase (CGTase) to produce an oligo-glycosyl N-substituted maronoline derivative of the formula: (wherein R has the meaning defined above and n is 0 or an integer from 1 to 24) and, if desired, reacting glucoamylase (GA) to form a compound of formula (II) wherein n is 0; (b) isolating the compound of formula (II); and (c) hydrolyzing the isolated compound of formula (II) to obtain an N-substituted moranoline derivative of formula (I). In accordance with another embodiment, the invention provides a stabilizing agent for an enzyme which comprises an enzyme stabilizing amount of an N-substituted moranoline derivative of the formula: (wherein R<1> is a hydrogen atom or, a C1-C5 alkyl, phenyl C1-C5 alkyl, phenyl C2-C5 alkylenyl, phenyl C2-C5 alkynyl phenoxy C1-C5 alkyl, phenoxy C2-C5 alkenyl or phenoxy C2-C5 alkynyl, wherein the phenyl moiety is unsubstituted or substituted); or a glucose oligomer thereof.