发明名称 |
IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS |
摘要 |
The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog. |
申请公布号 |
WO9106634(A1) |
申请公布日期 |
1991.05.16 |
申请号 |
WO1990US06449 |
申请日期 |
1990.11.07 |
申请人 |
BIO-TECHNOLOGY GENERAL CORP. |
发明人 |
BARTFELD, DANIEL;LIFSHITZ, RUTH;HADARY, DANY |
分类号 |
C12N9/02 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|