| 摘要 |
NEW MATERIAL:A substance having the following physical and chemical properties. Action: pyrimidine nucleoside + phosphoric acid base + ribose-1- phosphate. Substrate specificity: acting on pyrimidine nucleoside and bases except cytidine and cytosine. Optimum pH: 7-11. Stable pH: not being deactivated by treatment at 6.5-10.5 at 70 deg.C for 30 minutes. Optimum temperature: 70 deg.C. Proper temperature of action: stable even by treatment at pH7.0 at 50-70 deg.C for 60 minutes. Condition of deactivation: being deactivated by treatment at pH7.0-8.0 for 60 minutes. Molecular weight: 85,000 (gel filtration method by TSL gel G3,000 SW). Molecular weight of subunit: 54,000 (SDS electrophoresis), etc. USE:Useful for production of nucleic acid. PREPARATION:A bacterium such as Bacillus stearothermophilus JTS859 (FERM P-9666) belonging to the genus Bacillus is cultured. |
