发明名称 |
METHOD OF EXCISING AND PROPAGATING GENOMIC DNA |
摘要 |
A process for isolating, cloning and maintaining large genomic fragments of chromosomal DNA. This process includes the steps of introducing a marker into the chromosomal DNA to be cloned, inserting double-strand breaks into the chromosome followed immediately by combining the cells containing the chromosome with the fragmented marker to another cell line capable of harboring extrachromosomal DNA elements, and subsequently isolating genomic fragments by separating the extrachromosomal DNA from the chromosomal DNA of the other cell line. |
申请公布号 |
WO9105042(A1) |
申请公布日期 |
1991.04.18 |
申请号 |
WO1990US05574 |
申请日期 |
1990.09.28 |
申请人 |
THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW |
发明人 |
HAHN, PETER, J.;LANE, MICHAEL, J. |
分类号 |
C12N15/09;C07K14/47;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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