Genetically engineered eucaryotic host cells capable of expressing modified forms of eIF-2 alpha

摘要

Eucaryotic host cells are disclosed which contain a DNA molecule encoding an eIF-2 alpha mutant and preferably a DNA sequence encoding a desired heterologous protein. The DNA sequences are linked to expression control sequences permitting expression of the mutant eIF-2 alpha gene and the heterologous gene. Culturing such cells provides a method for the production of the desired heterologous protein. The mutations eliminate one or both serine residues at positions 48 and 51 of the eIF-2 sequence. In another aspect of the invention, the eIF-2 5'-untranslated sequence was observed to have effects on translation of heterologous mRNAs.