摘要 |
<p>Somatic hybrids of Rutaceae plants were obtained by allowing the protoplasts from embryogenic cells of a Rutaceae plant, which are of the nucellar origin, resistant to fusion treatment, and have a high proliferation activity, to fuse with the protoplasts of another Rutaceae plant having no proliferation activity, and then cultivating the fused protoplasts in a medium not containing a phytohormone but containing a saccharide in a concentration suitable for the selective embryogenesis of the hybrid cells.</p> |