| 摘要 |
PURPOSE:To obtain a packing agent which has excellent performances to separate a trace of containing component such as protein, etc. from a living body sample such as blood, urine etc. and to quantitatively determine it and also, is capable of fractionating at a high speed under low column pressure in case of liquid chromatography. CONSTITUTION:A packing agent for this liquid chromatography consists of a hydrophilic polymer containing a vinyl group monomer as a polymerization component. Consequently, the good separation capacity of components from an aqueous eluate is given. Further, this agent is formed by hollow and nearly globular particles. Hereby, in case of treating by the liquid chromatography after packing it in a column, a high-speed stream is obtained under low pressure and the component is separated at a high speed. Moreover, the ratio L/D of the particle diameter D to the thickness L of this agent at an optional place is set in a range of 0.06-0.45. In addition, a dent is hardly generated in the agent and a rise of pressure in the column is prevented when this agent is used in the column. |
