PRODUCTION OF ALPHA-GLYCOSYL RUTIN AND USE THEREOF

摘要

PURPOSE:To produce the subject compound on an industrial scale at a low cost by allowing a sugar transferase to act upon a rutin-rich liquid containing rutin and starch at high concentrations. CONSTITUTION:Rutin is dissolved at a concentration of >=0.5w/v% in an aqueous solution of an organic solvent containing methanol, etc., a starchy substance such as amylose is added to the solution in an amount of 0.5-50 pts. per 1 pt. of rutin, a sugar transferase such as alpha-amylase is added to the mixture and the components are made to react with each other at pH4.5-6.5 and 70-90 deg.C for 5-80hrs to obtain a reaction product containing a large amount of alpha-glucosyl rutin and a small amount of unreacted rutin and having a total rutin content of 1-20w/v% in terms of rutin. The reaction product is purified with a porous synthetic adsorbent resin and treated with beta-amylase to form alpha-glucosyl rutin and/or alpha-maltosyl rutin, from which alpha-glucosyl rutin is collected. As necessary, the rutin is used as an active component of a remedy for sensitive diseases.