VARIANT PROTEINS AND POLYPEPTIDES POSSESSING ENHANCED AFFINITY FOR IMMOBILIZED-METAL AFFINITY MATRICES

摘要

The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depend- ing on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.