| 摘要 |
To simultaneously determine a plurality of antibodies Aka1, Aka2, ...Akb1, Akb2, ... contained in a sample of human body liquid, the sample is introduced into a microtiter plate whose wells are coated with antigens Aga1, Aga2, ... complementary to one portion of the antibodies Aka1, Aka2, ... to be detected, and a plurality of solid supports, which are coated with antigens Agb1, Agb2, ... complementary to another portion of the antibodies Akb1, Akb2, ... to be detected are contacted with the sample; then, after complexing has been completed, the wells and the solid supports are washed free of non-adsorbed proteins, the complexes Aga1-Aka1 ... and/or Agb1-Akb1 ... adsorbed in the wells of the microtiter plate and/or on the solid supports are incubated with a mixture of labeled antigen enzymes Aga3E* ... Agb1E* ...complementary to the antibodies Aka1, Aka2, ... Akb1, Akb2, ... to be detected, and the activities of the enzymes Aga1-Aka1-Aga1E* ... Agb1 Akb1-Agb1E*, ... is photometrically determined upon reaction with a staining substrate.
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