| 摘要 |
<p>Methods of growing insect cells in sparged culture, particularly in airlift fermentors to high cell densities with high viability are herein disclosed. Further disclosed are media in which to grow airlift insect cell culture which media comprise a non-toxic protective agent, preferably a non-toxic water soluble polymer, and operating parameters, such as sparging rates and bubble sizes, that act to minimize cell damage and cell death from sparging. Further, the invention provides for methods and media for growing insect cells at large scale, for the production of wild type or recombinant viruses; for supporting the production therefrom of recombinant products, such as CSF-1 and IL-6, when the cells are infected by recombinant viruses; and for supporting the production therefrom of viral products, when the cells are infected with wild type viruses.</p> |
