| 摘要 |
<p>The present invention provides a cleavable conjugate whose linker contains a labile bond that is cleavable under a variety of mild conditions, including weakly acidic. Since the agent may be bonded directly to the linker, cleavage can result in release of native agent. The targeting molecule employed in the invention may be an intact molecule, a fragment thereof, or a functional equivalent thereof. In a particularly preferred embodiment, the targeting molecule is a monoclonal antibody directed towards a tumor-associated antigen in man. Another aspect of the invention provides a method for isolating a compound. The compound binds covalently to a solid phase which has been derivatized with the linker described above and is released in native form by a variety of mild conditions. An additional aspect of the invention provides a method for introducing into a compound a free sulfhydryl, amino, or hydroxyl group by use of a reagent structurally related to the linker described above.</p> |
