Method of regenerating plantlets from mesophyll protoplasts of phaseolus angularis

摘要

A method for regenerating plantlets from protoplasts isolated from mesophyll cells of abacterial seedlings of red bean (Phaseolus angularis). Protoplasts cultured in certain liquid media could divide continuously and many protoplast-derived calli may be obtained. In addition, various growth media containing the optimum concentration and the type of sugar, mineral salts, vitamins, and combinations and concentrations of several phytohormones for protoplast growth, division, callus formation and plantlet regeneration are provided.