| 摘要 |
<p>This invention is directed to novel oligonucleotides comprising at least one crosslinking agent and to the use of the resulting novel oligonucleotides for diagnostic and therapeutic purposes. The crosslinking agents of the invention are of the following formula (I'): R1-B-(CH2)q-(Y)r-(CH2)m-A', wherein R1 is hydrogen, or a sugar moiety or analog thereof optionally substituted at its 3' or its 5' position with a phosphorus derivative attached via oxygen to the sugar moiety by an oxygen and including groups Q1, Q2 and Q3, or with a reactive precursor thereof suitable for nucleotide bond formation; Q1 is hydroxy, phosphate or diphosphate; Q2 is =O or =S; Q3 is CH2-R', S-R', O-R', or N-R'R''; each of R' and R'' is independently hydrogen or C1-6alkyl; B is a nucleic acid base or analog thereof that is a component of an oligonucleotide; Y is a functional linking group; each of m and q is independently 0 to 8, inclusive; r is 0 or 1; and A' is a leaving group.</p> |
