| 摘要 |
PURPOSE:To obtain the subject protease, remarkably improved in thermal stability, excellent also in solubility and useful as a peptide synthetase by reacting a metal protease of the genus Bacillus with a specific acid anhydride and acylating the protease. CONSTITUTION:A metal protease of the genus Bacillus is reacted with at least one selected from succinic anhydride, maleic anhydride and acetic anhydride and acylated (e.g. under reaction conditions of pH7.5-9.0 and 15-30min) to afford the objective protease. |
