| 摘要 |
PURPOSE:To make DNA sepn. and detection with high accuracy and high sensitivity by imaging the light rays of individual images divided by prisms through a process, such as wavelength dispersion, onto a detector. CONSTITUTION:A gel plate 2 of plyacrylamide inserted between two sheets of glass plates 1 is irradiated with an argon laser from the side face. The light emitted upward from an irradiating part 3 is refracted by the upper prism 4 and is then imaged by a lens 6 onto the lower side of the two-dimensional detector 9. On the other hand, the light emitted to the lower part is refracted by the lower prism 5 and is then imaged again by the lens 6 onto the upper side of the detector 9. Namely, the DNA fragments which are the sample of fluorescence detection are fluorescence-labeled with four kinds of the fluorescent dyestuff of different emission wavelengths; for example, 515nm, 540nm, 573nm, and 610nm corresponding to four kinds of the terminal bases and, therefore, 4 pieces of lines are made in the upper part and the lower part. The discriminated detection of the four colors is, therefore, possible by using filters having transmission bands at two points of 515nm and 573nm for the upper filter 7 and 540nm and 610nm for the lower filter 8 as BPFs. |
