| 摘要 |
PURPOSE:To efficiently obtain the title enzyme useful for measuring anhydroglycitol, a diagnostic marker of diabetes by culturing a fungus belonging to Eumycetes, capable of producing anhydroglycitol dehydrogenase. CONSTITUTION:A fungus (preferably Pestalotia diospyri IFO-5282) belonging to Eumycetes, capable of producing 1,5-anhydroglycitol dehydrogenase, is cultured in a nutritive medium, the aimed enzyme is formed and accumulated in the culture cell and collected. The culture is preferably carried out under aerobic condition such as aerated spinner culture at pH5-7 at 25-30 deg.C. |
