Method for producing the MwoI restriction endonuclease and methylase.

摘要

The present invention is directed to a method for cloning and producing the MwoI restriction endonuclease by 1) introducing the restriction endonuclease gene from M. wolfei into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the MwoI restriction endonuclease activity, and 3) purifying the MwoI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the MwoI restriction endonuclease activity.