PURIFICATION OF HUMAN BCDF

摘要

PURPOSE:To readily and massively provide the subject substance purified in a high purity without containing endotoxin by subjecting a solution containing a human B cell differentiation factor produced by Escherichia coli to two step reverse phase chromatography. CONSTITUTION:A solution containing a human B cell differentiation factor (human BCDF) obtained from Escherichia coli is subjected to two step reverse phase chromatography to provide the purified human BCDF having a protein purity of >=99% and an endotoxin purity of <=0.6 endotoxin unit/mg protein. The crude human BCDF as the raw material includes a fused protein which is a human BCDF having a secretion signal peptide at the N terminal thereof.