Method for the detection of Gram-negative microorganisms, and DNA probes and antibodies suitable for this purpose

摘要

A method for the detection of Gram-negative microorganisms in which a DNA probe which is complementary to the nucleotide sequences coding for the enzymes lipid disaccharide synthase and/or CMP-KDO synthase, or DNA sections thereof which are 20 to 25 base pairs long, is used, or the nucleotide sequences, which code for the enzymes lipid A disaccharide synthase and/or CMP-KDO synthase, of the Gram-negative pathogens are isolated, cloned and expressed, and the expressed enzymes are isolated, the enzymes are used to immunise mammals, in particular mice, polyclonal antibodies are isolated from the immunised animals, or monoclonal antibodies are prepared in a conventional way, and the latter are used in a conventional way for the diagnosis, provides precise results and can be used for the diagnosis of infections with Gram-negative microorganisms.