METHOD FOR CULTURING CELL AND APPARATUS THEREFOR

摘要

PURPOSE:To suppress liquid flow so as not to increase the number of cells moving from the first to the second zones from that of the cells increasing in the first zone and carry out supply of nutrient sources and removal of waste materials for a long period by moving the nutrient sources and waste materials through a partition wall. CONSTITUTION:An apparatus is composed of an inner vessel part 102, divided with a cylindrical partition wall 104 having the closed bottom and suspending cells, an outer vessel part 103 for flowing a liquid and a jacket 105 for circulating warm water for keeping the temperature of the culture vessel 101 constant. The number of cells moving from the inner vessel 102 through the partition wall 104 to the outer vessel 103 is reduced. The liquid is fed from a culture medium feed pipe 106 to the outer vessel 103 with a pump 108 and taken out of the outer vessel part 103 through a taking out pipe 107 with a pump 109. The flow rates of the pumps 108 and 109 are changed manually or using a control computer.