| 摘要 |
A continuous method of quantitatively determining slowly reacting compounds with the use of a single measuring cell, the sample being injected into a continuously flowing carrier solution which is already provided with, or simultaneously with the sample is provided with reagent to form a sample zone in the form of a reproduceable gradient. Said sample zone is led into a measuring cell whereupon the flow is stopped by means of shunting the carrier flow, by stopping the pump, or by means of a valve. The reaction is allowed to take place in the measuring cell while a magnitude, characteristic for the reaction, is registered. The reaction speed can be detected from the incline of the obtained response curve and the amount of the sought-after compounds is calculated. By means of setting the stop at a certain point in the concentration gradient curve and always selecting said point at the same time distance from the peak of the gradient curve during flow, a total reproduceability of the analysis is ensured. |
