摘要 |
A method is described for the preparation of recombinant human beta interleukin-1 (beta hIL-1) in the homogeneous form, without endotoxins and with a specific activity of at least 1 x 10<8> Units/mg, by the purification, in a single chromatographic step, of the soluble cell extract obtained from Bacillus cells transformed by a replicable recombinant DNA molecule with expression containing the gene which codes for the protein. Beta hIL-1 thus purified can be used for stimulating the immune system of a patient, as an adjuvent in a vaccine, for the activation of repair mechanisms in cases of tissue damage, or in human therapy for the treatment of auto-immune diseases. |