METHOD OF SOMATOTROPIN SOLUBILIZATION AND NATURATION

摘要

METHOD OF SOMATOTROPIN
SOLUBILIZATION AND NATURATION
ABSTRACT
A method for solubilization and naturation
of somatotropin protein from refractile bodies of
host cells is disclosed. The method embraces the
discovery that an aqueous urea or dimethylsulfone
solution can be effectively used to solubilize
refractile bodies containing such somatotropin
protein. Once solubilized, somatotropin protein can
be natured in a urea or dimethylsulfone solution by
contacting the solution with a mild oxidizing agent
for a time sufficient to result in the formation of
the disulfide bonds. Naturation can efficiently occur
even at high protein concentration, in an impure
preparation and in the absence of reducing agent.