| 摘要 |
A method for increasing the yield of antigen-positive hybridomae and the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation of lymphocyte-containing cell populations, is described. In the method, lysosomotropic agents are caused to act in vitro on lymphocyte-containing cell populations for the removal of cell populations with a negative effect on the in vitro immunisation, whereupon the lymphocytes are in vitro immunised with thymus-dependent or thymus-independent antigens in the presence of a polyclonal activator. If a well-adjusted quantity of a polyclonal activator is used, it is possible to obtain an increase only in the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation without a lysosomotropic agent. A kit for use in in vitro immunisation of lymphocyte-containing cell populations is also described. Said kit comprises at least three containers, of which one holds lymphokines as active constituent, the second holds lysosomotropic agents as active constituent, and the third holds a polyclonal activator as active constituent. Another kit according to the invention does not have the container with lysosomotropic agents. |
