发明名称 |
Gene coding for insecticidal crystal protein |
摘要 |
Recombinant plasmids containing the larvicidal delta-endotoxin gene were constructed by inserting HindIII fragments of the Bacillus thuringiensis var. israelensis 72-75 Md plasmid into the Escherichia coli vector pUC12. Two recombinants producing a 27-kdal toxin (pIP173 and pIP174) were indentified by screening clones in an E. coli in vitro transcription-translation system. Both recombinants comprised pUC12 and common 9.7 kb HindIII fragment of the B. thuringiensis plasmid. The 27,340 Da polypeptide synthesized in vito from pIP174 transformed into E. coli JM101 and from B. subtilis 168 and spoOJ87 containing the 1.2 kb TaqI fragment from pIP173 was lethal to mosquito larvae.
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申请公布号 |
US4918006(A) |
申请公布日期 |
1990.04.17 |
申请号 |
US19860824912 |
申请日期 |
1986.02.03 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
ELLAR, DAVID J.;WARD, ELIZABETH S. |
分类号 |
C07K14/325;C12N15/70;C12N15/75 |
主分类号 |
C07K14/325 |
代理机构 |
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