摘要 |
PURPOSE:To prevent carry-over of a sample liquid even if there is no back sheath by providing a sample passage which is opened so that a part of a sheath passage is left in the upper and the lower parts of its opening part, in the sheath passage. CONSTITUTION:A sheath fluid 4 is led into a flow cell device 1 from two inlets 3. The sheath fluid 4 is brought to contraction in a passage 5, and flows into a constricted passage 6 in a laminar flow state. On the other hand, a sample fluid 9 flows into the device 1 from an inlet 8. The sample fluid 9 is brought to contraction in a passage 10, and flows into a confluence point 11 of the passage 5. In this case, since a part of the passage 5 is left in the upper and the lower parts of an opening 12, the sample fluid 9 is surrounded by the sheath fluid 4 from not only the right and the left directions but also the upper and the lower directions, becomes a thin flow 14 on the cross section center of the constricted passage 6 and flows. Since the thin flow 14 is a laminar flow, a particle selected by the sample fluid 4, for instance, a cell flows into a suspension one by one. |