VERFAHREN UND APPARAT ZUR BESTIMMUNG EINER NACHZUWEISENDEN SUBSTANZ, VERFAHREN ZUR EICHUNG EINES SOLCHEN APPARATES.

摘要

A method and apparatus are described for the determination of an analyte in an aqueous liquid while eliminating the biasing effect of unknown interferents which are preformed or formed in situ during the determination. The method comprises physically contacting a sample of the liquid with an interactive composition for the analyte; measuring the spectrophotometric responses generated by such contact at a primary wavelength lambda 1 and one or more secondary wavelengths lambda 2, lambda 3, . . . lambda n; and determining analyte concentration or activity using the equation: C=a0+a1[A1+ alpha 1A2+. . . + alpha n-1An] wherein C is analyte concentration or activity, A1, A2, . . . An are the spectrophotometric responses observed at lambda 1, lambda 2, . . . lambda n, respectively; and a0, a1, and alpha 1, alpha 2, . . . alpha n-1 are constants determined according to a calibrating method, also described herein. Such calibrating method is an empirical means for determining and recording in a chemical analyzer the a0, a1, and alpha 1, alpha 2, . . . alpha n-1 constants essential for making the analyte determination.