发明名称 |
IMMUNOASSAYS FOR CATALYTICALLY-ACTIVE, SERINE PROTEASES |
摘要 |
Methods for detecting and/or quantifying catalytically-active, serine proteases in a biological fluid are disclosed. The methods are useful for measuring the active enzymes of the coagulation/fibrinolytic system and evaluating the system or components of the system as indicative of thrombosis-related disorders. The methods involve the combined use of halomethyl ketone probes having broad specificity for catalytically-active serine proteases and immunological reagents specific for serine proteases of particular types. The halomethyl ketone probes are active site specific; they are only incorporated into catalytically-active serine proteases. An antibody is used to provide specificity for the particular type of serine protease. By the combined active-site-specificity of the halomethyl ketone probes and the type-specificity of the antibody, the catalytically-active fraction of a particular serine protease is determined. |
申请公布号 |
WO9003577(A1) |
申请公布日期 |
1990.04.05 |
申请号 |
WO1989US04192 |
申请日期 |
1989.09.26 |
申请人 |
THE UNIVERSITY OF VERMONT AND STATE AGRICULTURAL C |
发明人 |
MANN, KENNETH, G.;WILLIAMS, BRADY;TRACY, RUSSELL, P. |
分类号 |
G01N33/573;G01N33/86;(IPC1-7):G01N33/573;C12Q1/38;G01N33/543;G01N33/532 |
主分类号 |
G01N33/573 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|