SUPPORT-BOUND OLIGONUCLEOTIDES

摘要

A method of preparing an oligonucleotide bound to a support, such as porous glass or glass beads, involves attaching a nucleoside 3'-reagent through the phosphate group by a covalent phosphodiester link to aliphatic hydroxyl groups carried by the support. The oligonucleotide is then synthesised on the supported nucleoside, and protecting groups removed from the synthetic oligonucleotide chain under standard conditions which do not cleave the oligonucleotide from the support. Suitable reagents include nucleoside phosphoramidite and nucleoside hydrogen phosphonate.