Microfiltration apparatus & method of using the same.

摘要

The present invention provides a disposable manifold plate for use in conjunction with a conventional 96 well microtiter plate. The manifold plate has 96 wells formed therein and closed at the bottom by a plate or plates with provision for withdrawal of filtrate by vacuum. Each manifold plate has a self-contained vacuum manifold. Depending from the lower surface of each well bottom plate are a plurality of skirt members, each skirt member being axially aligned with one of the wells of the manifold plate and having formed therein a filtrate discharge passage. The skirts are of such a length that they will enter into the collection wells of a conventional multiwell collection plate at a point approximately 1-2 mm below the lip or upper surface of the collection plate thus enabling the separate collection of filtrate from each of the various wells of the manifold plate. The skirt means also functions as a guide for receiving a punch. At the completion of filtration the manifold plate may be inverted and, by use of the punch, a circular section of the filter medium is removed from each well, along with a circular blank cut from the bottom of the well bottom plate. This apparatus and method is applicable to a wide range of biological and biochemical analyses.