| 摘要 |
<p>A method of detecting, identifying and/or quantitating nucleic acids in a sample through determination of agglutination or inhibition of agglutination of suspendable particles having a member of a specific binding pair bound thereto is described. The member of the specific binding pair can be bound directly to the particle surfaces or attached through a spacer molecule which can, in turn, be either covalently bound or adsorbed to the particle surfaces. The suspendable particles are small enough to remain in suspension and will generally have a large particle size relative to the molecular weight of the DNA or RNA which is being determined. The presence or absence of nucleic acid sequences in a sample is determined by detecting agglutination of particles having a member of a specific binding pair bound thereto which becomes crosslinked via nucleic acid sequence complex labelled with at least two molecules of one member of a specific binding pair spaced an appropriate distance apart that the complex can act as a bridge between at least two particles labelled with a second member of a specific binding pair.</p> |
