<p>Selection of Streptomyces viridochromogenes DSM 4112 using phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistant selectants. Subjecting the total DNA of these selectants to cutting with Bam HI, cloning of a 4.0 kb fragment and selection for PTT resistance result in the DNA fragment which harbours the phosphinothricin (PTC)-resistance gene. The latter is suitable for producing PTC-resistant plants, and as resistance marker and for the selective N-acetylation of the L form of racemic PTC. <IMAGE></p>
申请公布号
EP0257542(A3)
申请公布日期
1990.03.07
申请号
EP19870112023
申请日期
1987.08.19
申请人
HOECHST AKTIENGESELLSCHAFT
发明人
STRAUCH, ECKHARD;WOHLLEBEN, WOLFGANG, DR.;ARNOLD, WALTER;ALIJAH, RENATE;PUHLER, ALFRED, PROF. DR.;WOHNER, GERHARD, DR.;MARQUARDT, RUDIGER, DR.;GRABLEY, SUSANNE, DR.;BRAUER, DIETER, DR.;BARTSCH, KLAUS, DR.