| 摘要 |
PURPOSE:To improve absorption in intestinal tracts and nutrient effects by hydrolyzing an aqueous solution of a protein with an endopeptidase and dipeptidyl carboxypeptidase(DPCP). CONSTITUTION:A culture obtained by culturing Bacillus subtilis HL521, strain, etc., is extracted and purified to afford DPCP having the following properties. Action; liberating peptides in dipeptide units of amino acids from the carboxy terminals of proteins. Optimum pH; 6.0-11.0. Action temperature; about 50 deg.C. Molecular weight; 110000 measured by a gel filtration method, etc. Endopeptidase, a proline-specific endopeptidase, derived from Flavobacterium meningosepticum, etc., and the above-mentioned DPCP are then added to an aqueous solution of soybean protein, etc., to carry out hydrolytic reaction at 25-60 deg.C for 8-72hr and afford a hydrolyzed solution. The resultant hydrolyzed solution ts subsequently purified to produce a low-molecular peptide composition having <=1000 molecular weight. |
