| 摘要 |
PURPOSE:To accomplish growth of Actinomyces by nicking the chromosome DNA of Streptomyces mycarofaciens with a restriction enzyme BamHI to insolate a midecamycin-tolerant gene-contg. DNA fragment. CONSTITUTION:The whole DNA is extracted, through e.g. SDS-phenol technique, from Streptomyces mycarofaciens (or its mutant) as a midecamycin-productive bacterium. The resultant DNA is nicked with a restriction enzyme BamHI to obtain a DNA fragment containing the objective gene together with a variety of DNA fragments. Thence, the resulting DNA fragment mixture is incorporated into a plasmid appropriate as a vector followed by application of a gene recombination technique to transform host bacteria using the above-incorporated product, thus obtaining numerous fragments containing the objective gene. |
